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Wellcome Trust Sanger Institute chooses NEBNext reagents of New England Biolabs for RNA sequencing
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Ipswich, Massachusetts
January 31 , 2019
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The Wellcome Trust Sanger Institute is one of the world's leading genome
centers, and through its ability to conduct research at scale, it is
able to engage in bold and long-term exploratory projects that are
designed to influence and empower medical science globally. Institute
research findings, generated through its own research programmes and
through its leading role in international consortia, are being used to
develop new diagnostics and treatments for human disease. The Institute
has one of the largest DNA sequencing facilities in the world,
processing DNA, RNA and tissue samples.
"The Wellcome Trust
Sanger Institute has recently re-evaluated its RNA-seq library
preparation methods and has chosen to implement the NEBNext Ultra II
Directional RNA Library Prep Kit," stated Mike Quail, Senior Staff
Scientist, R&D Sequencing at the Sanger Institute. "Our previous
RNA-seq library preparation required a minimum input of 500 ng total
RNA, but the high efficiency of the NEBNext Ultra II kit has allowed us
to reduce this to either 100 ng for a standard pipeline, or down to just
5 ng for a low input option, enabling RNA-seq to be carried out from
just 5,000-10,000 cells."
"When processing a large number of
samples, factors such as automation compatibility, workflow speed, input
amount and library quality become critical for efficient and successful
sequencing runs. Further, library preparation ideally needs to be
achieved with minimal optimization," stated Fiona Stewart, NEBNext
Product Portfolio Manager at NEB.
New England Biolabs, aimed to
address these challenges when developing their recently launched NEBNext
Ultra II RNA Library Prep kits for Illumina sequencing. "We know from
many of our customers that RNA samples can be problematic for a variety
of reasons. Library quality is often affected by low input amounts, RNA
quality, and the presence of contaminants. Further, workflows must
ideally be streamlined and optimized for a broad range of input
amounts," continued Stewart. "We kept these needs in mind when
developing the NEBNext Ultra II RNA Library Prep kits, and have been
able to make dramatic improvements on all fronts. We are pleased that
the Sanger Institute is having success with this kit for its samples and
workflow needs, and look forward to working together."
With the
NEBNext Ultra II kit, each step in the RNA library prep workflow has
been optimized, resulting in streamlined, automatable workflows for a
broad range of input amounts (5 ng – 1 µg). Kits are available for
directional (strand-specific, using the "dUTP method") and
non-directional library prep, and are compatible with poly(A) mRNA
enrichment or rRNA depletion.
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